The artists of the Renaissance period: Leonardo da Vinci (Link Neal), Donatello (Rhett McLaughlin), Michelangelo (Ian Hecox), and Raphael (Anthony Padilla); battle the Teenage Mutant Ninja Turtles: Leonardo, Raphael, Donatello and Michelangelo (voiced by Nice Peter and EpicLLOYD, performed by EpicLLOYD).
| Name | Type | Role | |
|---|---|---|---|
| Rhett McLaughlin | Guest Star | ||
| Ian Hecox | Guest Star | ||
| Anthony Padilla | Guest Star | ||
| Xin Wuku | Guest Star | ||
| Link Neal | Guest Star | ||
| Nice Peter | Director |