Six complete strangers with widely varying personalities are involuntarily placed in an endless maze containing deadly traps.
The film starts with a young woman named Becky (Greer Kent), shown to be trapped in the Cube. She enters another room, but unknown to her, the room has reversed gravity, as she is pulled up, and it is implied that she is killed.
A man is trying to escape from the titular Cube. Upon entering a trapped room, he is sprayed with a liquid that rapidly melts his entire body. The rooms in the Cube are being monitored from a remote observation room by two technicians, Eric Wynn and Dodd, who are unaware of who their employers are. The pair are shown playing chess during work, whereupon Wynn demonstrates mental calculator abilities which he uses to predict Dodd's moves. Wynn has also a hobby of drawing comics that portray him and Dodd as superheroes.