Alice, a former security specialist and covert operative, battles the Umbrella Corporation, an international pharmaceutical company whose bioweapons have triggered a zombie apocalypse.
At a genetic research facility called the Hive, a horrific viral outbreak occurs and a supercomputer seals the facility. It is up to the survivors, a speciality military unit, led by Alice and Rain to fight the powerful, out-of-control supercomputer and hundreds of scientists who have mutated into flesh-eating creatures to kill the undead, who if they can't be stopped, will overrun the Earth.
After narrowly escaping the horrors of the underground Hive facility, Alice is quickly thrust back into a war raging above ground between the living and the Undead. As the city is locked down under quarantine, Alice joins a small band of elite soldiers, led by Valentine and Carlos, enlisted to rescue the missing daughter of Dr. Ashford, the creator of the mutating T-virus.
Alice wakes up disoriented in a mansion. Wandering through its halls, she is forced to escape several Booby trap. However, she is eventually killed by a bounding mine hidden in the floor. Her body is dumped into a pit filled with dozens of Alice copies and is revealed to be one of many clones being produced inside of an underground facility whose entrance above ground is surrounded by thousands of zombies.
In a world ravaged by a virus infection, turning its victims into the undead, Alice, continues on her journey to find survivors and lead them to safety. Her deadly battle with the Umbrella Corporation reaches new heights, but Alice gets some unexpected help from an old friend. A new lead that promises a safe haven from the undead takes them to Los Angeles, but when they arrive the city is overrun by thousands of undead - and Alice and her comrades are about to step into a deadly trap.
Alice and the others on the Umbrella Corporation freighter ''Arcadia'' are attacked by a fleet of tiltrotor Bell Boeing V-22 Osprey led by Alice's former ally, Jill Valentine, resulting in the killing of many of the survivors in the ship. Alice is captured during the attack while the fates of Chris Redfield, Claire Redfield and K-Mart are left ambiguous.
Alice is the only survivor of what was meant to be humanity's final stand against the undead. Now, she must return to where the nightmare began - The Hive in Raccoon City, where the Umbrella Corporation is gathering its forces for a final strike against the only remaining survivors of the apocalypse.