Young Jun comes home from school to find a package waiting for him. He opens it, and although he is no enthusiast, he finds the most well crafted antique style doll he has ever seen. It's when he winds up its key, however, that everything begins. Destructive sister dolls start their own little drama, flying in and out of his poor, repeatedly broken window, a weepy sister doll comes crying for help, a crazy disembodied, a crazy sister doll comes body snatching... it's all a little much for Jun, but he is an affirmative action kind of guy, and he's not the type to ignore "people" who need his help, so he dives in.
ひきこもりの少年・桜田ジュンの元に「まきますか まきませんか」とだけ書かれたダイレクトメールが届いた。《まくこと》を選択したジュンの元へ届けられたのは第５のローゼンメイデン真紅。
そしてジュンの前に次々と現われる個性豊かなドールたち。なし崩しに真紅のしもべとなったジュンは、ローゼンメイデンの命とも言える《ローザミスティカ》を奪い合うアリスゲームに巻き込まれる。
某日，家裡蹲國中生的櫻田純，收到了一封廣告信「要上發條嗎 不上發條嗎」。選擇了「要上發條」的純，收到了寄來他家的第五人偶──真紅。性格各異的薔薇少女接連出現。被迫成為真紅僕人的純，捲入了為爭奪薔薇少女生命之源──「薔薇聖母」而展開的「愛麗絲遊戲」
