A man awakens to find he's been altered into a half machine by mysterious doctors. He escapes and finds 8 others like him who band together to fight the evil organization responsible for this inhuman act against them.
The cyborgs are no longer in duty, they live normal lives until a misterious space ship lands on earth, the only survivor on the ship warns about an evil conqueror called Zoa that wants to conquer the universe, the cyborgs are back in action..
27 years have passed since the last mission of the Cyborgs and the world seems to have forgotten them by now, but the missile attacks on the metropolis of Shanghai, the scene with which the film opens, awaken fear and the nine Cyborgs are recalled for what could be the their most dangerous and fundamental mission, to find out how and by whom these attacks were launched.
ミュートス・サイボーグとの戦いを終えたジョー。ささやかな休息もつかの間、イワンが“悪魔"の出現を警告した。調査を始めるゼロゼロナンバーたち。 一方、飛鳥了と共にデーモンハンターとしてデーモン族と闘うデビルマン《不動明》も敵の新たな動きを感じていた。互いに事件を追い、遂に遭遇するサイボーグ009とデビルマン。 誰も想像できなかった改造人間(サイボーグ)と悪魔人間(デビルマン)の本気バトル。対決のゴングは打ち鳴らされた!
引退したゼロゼロナンバー達の元へ、ジャーナリストのルーシー・ダベンポートが訪れる。彼女は迫る危機を告げ、一同に再び立ち上がるように頼むが…。
