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Yes

Josh Keaton as Spectacular Spider-Man
Honoka Inoue as Kaoruko Waguri
Jorma Taccone as Adriano Tumino
Loraine Velez as Maria
Sara Wiseman as Dar
Britt Irvin as Raquelle (voice)
Jharrel Jerome as Miles G. Morales
Justin Roiland as Korvo
Natsuki Hanae as キーフリー / Qifrey
Dee Bradley Baker as Animal Voices (voice)
Diedrich Bader as Tank 'The Shredder' Evans (voice)
Hiroki Yasumoto as Qual
Ben Schwartz as Sonic the Hedgehog
Elizabeth Perkins as May
Arthur Ortiz as Additional Voices (voice)
Marina Inoue as Yoko Littner
Hannah Kaiser as Tall Ghost Girl (voice)
Kenjiro Tsuda as Gordon
Matthew Yang King as Luka
Metro Boomin as Metro Spider-Man
Roger L. Jackson as Mojo
John Hodgman as Charlie Jones / Other Father (voice)
Stephen Colbert as President Hathaway (voice)
Peter Sohn as Ganke

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