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Yes

Yoji Ueda as Eisen
Michael Daingerfield as Chunk Munchers Cereal / Light Bulb / Indian Chutney (voice)
Angela Lansbury as The Dowager Empress Marie (voice)
James Cromwell as Professor Robert Callaghan (voice)
Rie Takahashi as Yuzuriha
Hiroshi Kamiya as Jinpachi Ego
Bill Hader as Firewater / Tequila / El Guaco (voice)
Harland Williams as Ketchup / Drug Dealer / Baba Ganoush (voice)
Nicole Oliver as Sally Bun / Ice Cream / Watermelon / Female Shopper #1 (voice)
Seth Rogen as B.O.B. (voice)
Alistair Abell as Mariachi Salsa / Gefilte Fish (voice)
Katie Griffin as Alex
Cody Taylor as James Aohn / Alina Loud / Zach Gurdle
Karl Swenson as Merlin (voice)
Sumi Shimamoto as Toki (voice)
Julie Andrews as Marlena Gru (voice)
Amy Poehler as Eleanor (voice)
Rikiya Koyama as Joichiro
Lupita Nyong'o as Roz
Koki Uchiyama as Izumi Miyamura
Ami Koshimizu as Miko Kuroda
Stephanie Beatriz as Vaggie
Bill Burr as Nightclub Caller (voice)
Wataru Katou as Reno Ichikawa

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